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A retrospective evaluation of a multiplex polymerase chain reaction test directly applied to blood for the management of sepsis in the critically ill

S Omar, S Murphy, R Gheevarghese, N Poppleton

Abstract


Background. Blood culture (BC) is the established gold standard for microbiological diagnosis of bloodstream infection (BSI); however, its sensitivity is poor.
Objectives. The primary objective was to determine the sensitivity and specificity of the Magicplex Sepsis Real-time Test, a multiplex polymerase chain reaction test (mPCR), and BC to detect BSIs. Secondary outcomes included determining the prevalence of BSIs.

Methods. A retrospective review of a technical evaluation of the mPCR. Patients requiring BC had a blood sample collected for mPCR.
Results. The respective sensitivity and specificity of mPCR for the detection of BSI were 50% (n=7/14) and 58% (n=18/31), while the sensitivity and specificity using BC were 36% (n=5/14) and 68% (n=21/31), respectively. The addition of mPCR to BC increased BSI detection during sepsis from 36% to 64%.
Conclusion. The use of mPCR directly applied to blood may increase the detection of micro-organisms associated with BSIs in critically ill patients requiring BC investigation.


Authors' affiliations

S Omar, Critical Care, School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa

S Murphy, Critical Care, School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa

R Gheevarghese, Critical Care, School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa

N Poppleton, Critical Care, School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa

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Cite this article

Southern African Journal of Critical Care 2021;37(3):115. DOI:10.7196/SAJCC.2021.v37i3.495

Article History

Date submitted: 2022-01-28
Date published: 2022-01-28

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